In this study, inhibitory effect of SO on elastase activity was determined according to the previously described method. SO showed the elastase inhibitory effect in a dose dependent manner. SO was found to inhibit elastase activity highly at a concentration of 10 mg/ml. The production of Reactive oxygen species (ROS) is induced by NE13,14). They reported that NE enhancement of MUC5AC messenger RNA levels was dependent on the production of intracellular oxidants or an alteration in the redox state of the cell. It means that ROS may play a role in elastase mediated inflammation. Accordingly, anti-oxdative activities of SO were also examined. DPPH free radical scavenging capability of SO was measured at each concentration (0, 4, 20, 100, and 500 mg/ml). A dose dependent free radical scavenging capability was observed. Nitric oxide plays a pivotal role in elastase mediated diseases15,26). So, nitrite scavenging activities were also examined. However, nitrite scavenging capability changes at various pH environments. Accordingly, nitrate scavenging activities at pH 1.2, 3.0, and 6.0 were measured in this study. Considering data at pH 1.2, 3.0, and 6.0, nitrite scavenging capability was varied with increasing pH, suggesting it is pH dependent. The low pH environments (1.2 - 3.0) could be the best environment for nitrite scavenging activity of SO. However, pH 6.0 did not show the nitrite scavenging capability at all. In conclusion, SO showed the inhibiting effects on the elastase, and free radical scavenging capability of DPPH and nitrite. These results suggest that SO may have potential effects for pulmonary emphysema and pulmonary hypertension. Further studies might be needed to unravel exactly under the clinical trial and mechanisms.